Evaluation of Canine Adipose-derived Stem Cells in a Healthy Mice Subcutaneous Model

Canine adipose-derived stem cells (cASCs) have great interest for cell-based therapies in Veterinary Medicine. As the behaviour of these cells in non-autologous recipients is not deeply characterized, it is mandatory to study them in new animal models previously to canine specie. In this work, cASCs were injected subcutaneously in mice and these cells were detected by immunohistochemistry using vimentin, CD44 and keratin. The local response evaluated by histology did not reveal signals of significant inflammatory reaction neither in the lymph nodes or other organs. This study showed the implantation of cASCs induced a scarce inflammatory response. Hsd:CD1 (ICR) mouse can be proposed as an animal model to study the in vivo behavior of the cASCs and to validate new cASCs-based approaches avoiding or reducing the use of dogs in research. Evaluation of Canine Adipose-derived Stem Cells in a Healthy Mice Subcutaneous Model João Filipe Requicha1-3*, Pedro Pires de Carvalho1,3, Maria dos Anjos Pires1,4, Maria Isabel Dias2,3, Rui Reis3, Manuela Estima Gomes3 and Carlos Viegas2,3 1Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal 2Lusófona University, Avenida do Campo Grande, Lisboa, Portugal 33B’s Research Group Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal 4CECAV Centre for Studies in Animal and Veterinary Sciences, Department of Animal Sciences, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal *Corresponding author: João Filipe Requicha, Faculty of Veterinary Medicine, Lusófona University, Avenida do Campo Grande 376, 1749-024 Lisboa, Portugal, Tel: +351 967 793 272; E-mail: [email protected] Received March 06, 2016; Accepted August 23, 2016; Published August 31, 2016 Citation: Requicha JF, de Carvalho PP, dos Anjos Pires M, Dias MI, Reis R, et al. (2016) Evaluation of Canine Adipose-derived Stem Cells in a Healthy Mice Subcutaneous Model. J Stem Cell Res Ther 6: 358. doi: 10.4172/21577633.1000358 Copyright: © 2016 Requicha JF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.